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Binding kinetics and thermodynamics, autophagy induction, apoptosis, and self-renewal were evaluated via isothermal titration calorimetry, Western blotting, annexin V assays, and tumorsphere formation assays, whereas aptamer-cell interaction and intracellular drug accumulation were quantified using flow cytometry

In patients with gallstones, vitamin C was shown to influence the environment of the gallbladder, resulting in a higher concentration of phospholipids, along with a changed ratio of bile acids, indicating an influence of vitamin C on the formation of gallstones

Natural vitamin E appears to be better utilized than synthetic

doi: 10.1007/s00277-005-1026-4

pH 7.4) for 96 h at 4 C